The DNA templated silver nanoclusters were prepared by reducing silver nitrate with sodium borohydride as reducing agent to detect HBV sequence. The factors influencing the fluorescence of silver nanoclusters were explored. Under the optimized experimental conditions, the HBV sequence was analyzed with 466 nm as excitation wavelength. The probe had a good selectivity for HBV sequence. There is a good linear relationship between 0.05 μM and 1 μM and limit of detection is 0.016 μM.

The industrial boiler is more important in the national economic production, in which the fixed discharge and continuous discharge of the boiler is an indispensable process to ensure the normal operation of the boiler. With the implementation of the fixed discharge and continuous discharge process, a lot of steam will be lost. In this paper, waste steam recovery equipment and automatic control system are used to recover waste heat from excess steam, and the recovered heat is used in production and life to avoid energy waste.

This paper introduces a data exchange technology between Simulink and Kingview software based on OPC service, and illustrates the establishment process of client and server through simple examples, and conducts simulation experiments. The results show that through OPC technology, the real-time exchange of data between different platforms is basically completed, and it can be seen from the curve that the effect is good.

There are many problems in the four-tank system, such as the model's uncertainty, slow dynamic performance,and serious coupling.Aiming at these problems, this thesis designs a fractional-order sliding mode control algorithm to control the water tank's liquid level. Specifically, the traditional integer-order sliding mode surface and the approaching rate are improved to obtain a fractional-order sliding mode surface and approaching rate with more flexible control effects. Moreover, Lyapunov Theory is applied to theoretically analyze the asymptotic stability conditions, application range, and characteristics of the fractional sliding mode controller, and the algorithm design method is proposed. Through the comparison with the fractional PID controller, the simulation experiment reveals that the fractional sliding mode controller possesses good dynamic performance for the expected value of the given liquid level, can effectively reduces the overshoot in the control process, and has good robustness to the perturbation of system parameters.

In view of the basic flow and control requirements of the acetaldehyde reaction process,the reactor temperature control and pressure control methods were studied, and the acetaldehyde reaction process control system based on Siemens S7-300 PLC was designed.Using WinCC configuration software to design the man-machine interface screen, realize the centralized monitoring and automatic control of the acetaldehyde reaction process,effectively improve the production efficiency of acetaldehyde ,and reduce the production labor force.

Methanol is an important basic raw material for chemical industry. Methanol recovery project is of great significance to the construction of environmental protection projects and the improvement of China's modern industrial system. In a petrochemical company VOCx exhaust gas treatment project of methanol washing process as the research background, design based on ABB company AC800F DCS automatic control system for the methanol washing, after the system was put into operation, effectively improve the efficiency of the methanol washing recovery process, reduces the worker labor intensity, good results have been achieved in actual production.

With the deepening of the information construction of colleges and universities, the traditional office mode has been unable to meet the needs of the modern office, office of information technology in the mode of combining the popularity The application of information technology at present, will gradually replace traditional redundancy heavy and complicated part of the job title appraisal work of colleges and universities is one of the important steps in the process of training qualified personnel of institutions of higher learning, its review workload big and complex Therefore, the use of informatization evaluation way, can efficiently improve the inspection efficiency In this paper, based on Web technology, using Thinkphp framework and MySql database design for professional colleges and universities the voting system Through practical application, compared with the traditional evaluation method, the effect is significant, which greatly improves the work efficiency and provides a good idea for the informatization of professional title evaluation in colleges and universities.

In order to effectively improve the operability and scalability of wearable device users, a low-cost wearable system devices based on multiple sensors and supporting cloud services is designed. Each node of the system consists of 5 sensors and a micro-controller Arduino Uno. The device in this paper includes microcontroller Arduino uno and five sensors, which are heart rate sensor, respiratory sensor, stress anxiety sensor, body temperature sensor and blood glucose sensor. Body attribute data monitored by the sensors is sent to the application in real time through MQTT protocol. These data are stored in the cloud for communication between doctors and patients. The most important feature of the proposed system design is the effective combination of cloud computing and wearable technology, which can achieve the monitoring and management of the patient's health status, and the operation is simple and convenient, the operability is strong. At the same time, the software and hardware of the system is feasible, which has the advantages of low cost and large area popularization.

To make the operation of visible indoor positioning simpler and independent of line-of-sight view and position registration, photodiodes are used as the receivers, and the system is optimized in the hardware by assembling compatible photodiodes and compact LED. In the algorithm part, based on fingerprint technology, the Gauss Process Regression (GPR) algorithm is used for location. Firstly, the GPR environment is modeled and a strength distribution model is constructed. Then, Bayesian theory is used to obtain the expression of location. The experiment is carried out in a 3m×3 m platform area and 25 targets are tested. Compared with similar indoor positioning systems, the proposed system has higher positioning accuracy and achieves centimeter-level positioning. The proposed system can accurately locate moving targets for a long time and has good robustness.

Today's world is a highly competitive era for network application development. Accurate, economical and efficient development and application are the needs of the times.The design pattern of MVC architecture is widely used in the design of application systems at present. based on MVC framework, this paper optimizes the traditional MVC design pattern by using Spring boot framework and the concept of "Convention is better than configuration".  Solve the limitation of traditional MVC design pattern.

Aiming at the problem that the actual response time of the existing data analysis system is too long, a data analysis system of College Students' physical fitness test is designed under the support of association rules. Build the hardware structure of the system, design the data acquisition circuit, control the power output of the hardware circuit, optimize the data acquisition process of College Students' physical fitness test; This paper constructs the association rules of physical health, uses the dichotomy model to control the function threshold, and combines Java technology to divide the college students' physical health data analysis system into login module, data management module, data analysis module and data callback module, determines the physical test data set, and generates the actual health data analysis rules, Complete the analysis of College Students' physical fitness test health data. The experimental results show that: after a data analysis, the number of distinguishable data items is equal to the actual health data items, and the system response time is the shortest.

At present, the simulation results of athletes' running training intensity are only a single screen, which leads to the low visibility and long simulation time of athletes' running training intensity, which are inconsistent with the actual results of athletes' running. For this reason, it is proposed to use the images reflected between frames to define the athletes' running training intensity. simulation. Use statistical measurement methods to obtain athletes’ physiological parameters, and calculate matching factors to match athletes’ physiological parameters with those of the simulated human; determine the simulated human’s joint rotation, rotation angle, running step length, pace, pace and speed on the simulation platform Athletes are consistent, calibrate the motion posture of the simulated person; substitute the calibrated motion posture data into the simulation platform, and use the images reflected between frames to form a continuous virtual animation of athlete running training, analyze the athlete's running training, and define the athlete's running training intensity. The experimental results show that under the same experimental parameters, the consistency between the simulation results and the real running motion model of the human body is detected. It is concluded that the proposed method can accurately simulate the athlete's running posture, with high visibility and short simulation time, which is consistent with the actual running movement of the athlete. The results are consistent, the simulation effect is good, and it can provide some help for supervising the athletes' running training process.

An automatic adjustable wheelchair is designed for the elderly. The back of the wheelchair is adjusted automatically by the incomplete gear mechanism.When the back is up, it can be used as a common wheelchair; when the back is flat, it can be used to lie flat, and people can move from the wheelchair to the bed and sofa through the roller of the back and seat.The wheelchair is stable in operation, reliable in performance and effective in function realization.

In the conventional oscillation fault diagnosis system, the noise of the collected oscillation signal is large, which leads to the low confidence of the fault category. In order to solve this problem, a fault diagnosis system for oil film oscillation of steam turbine based on cogeneration is proposed. Coupled with cogeneration production mode, the redundant exhaust steam of circulating steam turbine unit is used to increase the power generation of the system to complete the hardware design; the wavelet packet de-noising algorithm is used to eliminate the collected oscillation signal noise, decompose each time and frequency band of the signal, sort the time domain sub signal energy value in the frequency band, form the fault signal eigenvector, input the neural network, and output the oscillation fault after training The diagnosis mode completes the software design. The oil film oscillation signals under the conditions of full load, minimum load and noise are collected, and the comparative experiments are set. The results show that the designed system improves the reliability of fault diagnosis, and the fault diagnosis results are more accurate and reliable.

In recent years, the situation of water pollution is more serious, which has greatly damaged the ecological environment of our country, according to the relevant instructions of the State Council , “sewage treatment” is an urgent problem. In order to achieve efficient treatment of sewage, preparation of large amounts of ozone-based microbubbles is the researching clue, developed two kinds of array ozone-based microbubble generators, namely a Co-flowing array and a T-junction array; the bubbles preparation effect of two kinds of bubble generators was verified by experiments, by verification, it is concluded that the bubbles preparation effect of the improved Co-flowing array microbubble generators is more ideal; Use the improved Co-flowing array microbubble generators to carry out the following researches, thereby, which provides technical supports for efficient sewage treatments.

In view of the poor welding quality caused by the motion interference between the exhaust pipe and the welding fixture, and the life of the clamping mechanism reduced due to the unreasonable selection of the working cycle of the clamping mechanism. A four claw welding fixture is designed by CATIA software. The device is mainly composed of clamping mechanism and indexing mechanism. The model is imported into ADAMS simulation software for kinematics simulation, and the simulation results of displacement and angle of clamping mechanism and indexing mechanism, as well as the data results of motion speed, acceleration and displacement of four clamping claws in clamping mechanism under different working cycles are obtained. These results provide theoretical basis for solving the motion interference problem of welding fixture and reasonably selecting working cycle, thus the design cycle and cost of welding fixture are saved, and the reliability of welding fixture is improved.

A pneumatic flexible five-finger manipulator is designed, which uses single-drive and multi-drive unidirectional bending flexible joints as single-degree-of-freedom finger joints, which can perform multi-degree-of-freedom grasping, grasping, pinching and other flexible hand movements; build a kinematics experiment platform for the manipulator And the grasping experimental system, using the 3D motion capture system to obtain the movement space of the fingers of the manipulator, the manipulator can stably grasp objects of different shapes and qualities in daily life, which verifies that it has a grasping ability similar to that of a human hand. The experimental results show that the fingers of the manipulator basically fulfill the design requirements, realize their own grasping functions, and have good flexibility and adaptability.

Aiming at the shortcomings of poor flexibility and heavy weight of common linear drives on the market, a pneumatic flexible three-muscle axial drive has been designed and developed.The theoretical analysis of the driver is carried out to obtain the formulas of driving force and air pressure, elongation and air pressure, and an experimental platform is built to verify the formula of the driver.Analyzing the difference between the theoretical value and the experimental value and drawing a conclusion,。Studies have shown that when the extension of the axial actuator is constant, the greater the air pressure, the greater the driving force. When the air pressure of the axial drive is constant, the greater the elongation, the smaller the driving force.the research provides a certain basis for the control and use of the axial drive in the future.

Cellulose is one of the most important renewable resources in nature. In this paper, a strain j-n3 was isolated from the soil of corn field. The isolated medium with carboxymethyl cellulose as the sole carbon source and Congo red staining method were used for preliminary screening, and then the screened strains were cultured by liquid fermentation and enzyme activity was determined. Combined with morphological characteristics and molecular biological identification, strain j-n3 was identified as Bacillus.According to the preliminary study of enzymatic properties, the optimum pH of the enzyme is 6.0, and the optimum reaction temperature is 50℃. After treated with strain J-N3 for 20 days, the weight loss rate and cellulose decomposition rate of corn stalks reached 26.22% and 31.01%, respectively.

The financial performance evaluation is a very important part of the performance evaluation of  listed companies.  From the perspective of profit, debt repayment, operation, development and stock price, we selects 16 financial indicators to build an evaluation system for 22 listed companies in the brewing industry.  By use of the of analysis method, it aims to evaluate the financial performance of the selected object. According to the comprehensive factor score,  we analyze the overall financial situation of the companies.  Finally,  we give some investment suggestions.