碱性磷酸酶检测方法研究进展

doi:10.16039/j.cnki.cn22-1249.2019.05.002

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摘要

碱性磷酸酶（ALP）广泛的存在于人体内，它可以控制多种生物体内的生物过程，目前已经广泛地被的应用于临床、生物、医学等各个领域。作为一个与多种疾病有关的生物标志物和研究实验的主要工具酶，对ALP进行分析的经典方法已经不能满足新类型样品对灵敏度、精度、抗干扰性和广泛针对性的需求。因此，为了更好的研究检测ALP的方法，我们阐述了国内外最新的ALP提取方法和检测技术，同时将这些研究的主要优缺点进行了总结和比较。最后，我们对ALP检测技术的发展前景和研究趋势进行了探讨，希望为今后的工作提供一些启示。

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	李晓双
	张浩
	娄大伟

关键词: 碱性磷酸酶检测方法提取方法

Abstract:

Alkaline phosphatase (ALP) exists widely in human body and controls the processes in various physiological organisms. At present, it has been widely used in clinical, biological, medical and other fields. As a key biomarker of many diseases and a major tool of enzyme experiments, the classical ALP analysis methods cannot meet the new requirements of sensitivity, accuracy, anti-interference and wide range of targeted sample types. In order to better study ALP detection technology, this paper describes a variety of extraction and detection methods, including the latest ALP detection technology at home and abroad. At the same time, we summarize and compare the main advantages and disadvantages of these studies. At the same time, the future development prospects and trends are discussed, hoping to provide some inspiration for future work.

Key words: Alkaline phosphatase detection method extraction method

出版日期: 2019-05-25 发布日期: 2019-05-25 整期出版日期: 2019-05-25

ZTFLH:

O65

引用本文:

李晓双, 张浩, 娄大伟. 碱性磷酸酶检测方法研究进展 [J]. 吉林化工学院学报, 2019, 36(5): 6-11.
LI Xiaoshuang, Zhang Hao, LOU Dawei. Progress in Alkaline Phosphatase Detection . Journal of Jilin Institute of Chemical Technology, 2019, 36(5): 6-11.

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http://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2019.05.002 或 http://xuebao.jlict.edu.cn/CN/Y2019/V36/I5/6

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