龙胆泻肝汤的体外肝微粒体代谢研究

doi:10.16039/j.cnki.cn22-1249.2025.01.003

Journal of Jilin Institute of Chemical Technology, 2025, 42 (1): 11-22 doi: 10.16039/j.cnki.cn22-1249.2025.01.003

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Study on Liver Microsomal Metabolism in Vitro of Longdan Xiegan Decoction

ZHANG Ranran¹,ZHANG Yan^2*,YANG Xiudong^1*

(1.School of Chemical and Pharmaceutical Engineering, Jilin University of Chemical Technology, Jilin City 132000, China:2.School of Medicine ,Jiaxing University,Jiaxing 314001 .China)

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Abstract The aim was to investigate the transformation of drug components in Longdan Xiegan Decoction in liver microsomal metabolism model in vitro. Firstly, Gentiana manshurica Kitag, Bupleurum chinense DC, Scutellaria baicalensis Georgi, Gardenia jasminoides Ellis, Rehmannia glutinosa Libosch, Alisma orientale, Plantago asiatica L, Angelica sinensis (Oliv.) Diels, Aristolochia manshuriensis Kom and Glycyrrhiza uralensis Fisch were used to prepare the decoction, and the decoction was prepared by decocting, cooling, decocting, concentrating and vacuum drying. Secondly, liver microsomes were prepared from rat liver tissues, including liver lavage, centrifugation, filtration and precipitation, and finally liver microsomal enzymes containing 20% glycerol were obtained. Then, an in vitro liver microsomal enzyme incubation system was constructed, and microsomal protein, NADPH, magnesium chloride and substrate were added into the reaction system of 150 μL. Sampling operations were performed at different time nodes, and the samples were analyzed by HPLC and MS, respectively. HPLC results showed that with the extension of incubation time, some drug components were transformed and new metabolites may be formed. Gentiopicroside, geniposide, geniposide acid, baicalin, saikosaponin A and saikosaponin D were identified by MS analysis, and the possible metabolic pathway of baicalin was speculated. In summary, this study established in vitro liver microsomal metabolic model, combined with HPLC - MS technology, preliminatively simulated and studied the metabolic process of liver microsomes in Longdan Xiegan Decoction, and provided a reference for further exploration of its pharmacodynamic mechanism.

Key words： Longdan Xiegan Decoction liver microsome toxicity mechanism

Published: 05 July 2025

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Cite this article:

ZHANG Ranran,ZHANG Yan,YANG Xiudong. Study on Liver Microsomal Metabolism in Vitro of Longdan Xiegan Decoction[J]. Journal of Jilin Institute of Chemical Technology, 2025, 42(1): 11-22.

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https://xuebao.jlict.edu.cn/EN/10.16039/j.cnki.cn22-1249.2025.01.003 OR https://xuebao.jlict.edu.cn/EN/Y2025/V42/I1/11