In recent years, microbial metabolomics has gradually become a new analysis method. Although microorganisms have the characteristics of simple structure and single nutritional requirement, they also have many kinds of their own, and the physical and chemical properties of intracellular metabolites are different. Therefore, there are still a lot of deficiencies in the pre-sample treatment. Based on the analytical method of GC-MS, a metabolomics analysis method for yeast S288C was established in this paper. The effects of five solvents, including methanol (-20℃), acetonitrile/water (1:1) (-20℃), acetonitrile/methanol/water (2:2:1) (-20℃), water/isopropanol/methanol (2:2:5) (-20℃) and 75% ethanol (boiling) on the extraction of intracellular substances were investigated. The optimum solvent was water/isopropanol/methanol (2:2:5) (-20℃). The method could detect 348 metabolites, 142 metabolites are identified by NIST library searching, including 46 amino acids, 9 carbohydrates, 30 organic acids, 17 alcohols and 23 esters.

Alkaline phosphatase (ALP) exists widely in human body and controls the processes in various physiological organisms. At present, it has been widely used in clinical, biological, medical and other fields. As a key biomarker of many diseases and a major tool of enzyme experiments, the classical ALP analysis methods cannot meet the new requirements of sensitivity, accuracy, anti-interference and wide range of targeted sample types. In order to better study ALP detection technology, this paper describes a variety of extraction and detection methods, including the latest ALP detection technology at home and abroad. At the same time, we summarize and compare the main advantages and disadvantages of these studies. At the same time, the future development prospects and trends are discussed, hoping to provide some inspiration for future work.

Resveratrol is a kind of natural polyphenol compound with a high content in grapes, peanuts and other plants, and it has been demostrated to be a green anti- cancer drug. Resveratrol may inhibit tumor cells by anti- peroxidation, regulating redox metabolism of cells, interfering with autophagy and reducing cell invasiveness. In recent years, the effects of resveratrol on suppressing the proliferation, inducing the apoptosis and inhibiting angiogenesis of tumor cells have prompted a large interest from researchers, Resveratrol can not only induce the apoptosis of tumor cells via intrinsic or extrinsic pathways and cell cycle arrest, but also inhibit the migration of tumors via modulating collagen degradation related signal target. This paper reviews the research progress of the anti-tutor effect of resveratrol.

The intermediate o-nitrophenyl diazo salt was synthesized from o-nitroaniline and diazo salt. The compound 1, 2-nitro-4'-dimethylaminoazobenzene was further prepared by coupling diazo salt with N, N-dimethylaniline. The effects of reaction conditions such as pH value, stoichiometric ratio and temperature on the synthesis were investigated. The structure of the compound was characterized by infrared spectroscopy. The acid-base discoloration and spectrum properties of 1 were studied by ultraviolet spectroscopy. The experimental results show that 1 has high molar absorptivity (ε=2.42*10⁴ L/mol.cm) and good acid-base discoloration.

Flue gas desulfurization of coal-fired boilers is an important measure to control air pollution emissions and effectively prevent acid rain. At present, there are more than 200 flue gas desulfurization technologies researched and developed at home and abroad. Among them, wet flue gas desulfurization technology is widely used in coal-fired boilers due to mature technology and high efficiency. This paper analyzes two commonly used methods, gypsum desulfurization (to waste The technical process of desulfurization (for waste) with ammonia method, and the existing problems are analyzed and discussed.

FDM3D printers have been popular in various industries due to their cost advantages, but they also bring many problems due to their low printing accuracy. This paper designed a model for the Core XY structure for the desktop printing walking platform level FDM3D printer, the printer to print a higher space utilization, walking platform structure is more lightweight, minus the quality of the X axis stepper motor, thus greatly solve the printing process in the process of large inertia, in the face of small area print will be more careful, raise the precision of the printing.

In view of the problems of chocolate 3D printer such as single printing color, difficult continuous extrusion and low printing accuracy, a new design of chocolate 3D printer is proposed. The new 3D printer of chocolate has more high precision, easily continuous supplying materials, and what’s more, printed in two colors. In the design, a screw push rod is employed as the feed part to extrude raw materials, which can realize that the storage box can continuously transfer raw materials to the feed box. Heating devices are installed on both the feed box and the nozzle part. The PID algorithm is used to control the temperature of the device, which is conducive to maintaining the stabilization of the melting temperature of chocolate. To serve the needs of two-color printing, the nozzle is designed into two extrusion holes in one head. Each hole can be controlled the condition of opening and closing independently. For a purpose of improving printing accuracy, the feed mechanism is fixed on the rack, and the nozzle is fixed on the motion mechanism, which is helpful to reduce the motion inertia and improve the printing accuracy.

The deepening application of three-dimensional (3D) digital factory is one of the key problems currently encountered in domestic petrochemical enterprises. In this paper, we present a framework design of the intelligent operating optimization system for 3D digital factory by analyzing the operation optimization requirements of petrochemical enterprises in 3D digital factory circumstance. From three aspects of cross-platform bidirectional data transmission method, operation optimization simplified modeling and solution, and operation optimization system digital platform integration, the solving strategies for different technical difficulties are discussed in detail. By combining with the current information system in petrochemical enterprises, this framework can realize the production operating optimization on the platform of 3D digital plant, which makes it possible to explore effective solution for the implement of production operating optimization in the digital factory environment.

Aiming at the problem of low control precision during robot operation in industrial production process, the self-learning and adaptive intelligent control method--the neural network is used for optimal control, and the control precision of the whole operation process is optimized by the trained network. Thereby improving control accuracy and improving efficiency.

This topic mainly using the multi-parameter controlled electronic double CCD imaging system to implement the LLL image acquisition, the computer sent instructions to control a variety of camera parameters and a variety of work mode, complete the electronic double CCD sensor chip parameters testing and processing, to obtain the required power supply stability, low noise, good image image data.

The characteristics of air quality evolution in Jilin City during the heating period were studied by using the real-time atmospheric environmental monitoring data released by Jilin Environmental Protection Monitoring Center. The trend of monthly average mass concentration of   articulate matters (PM10), PM2.5,SO2, NO2, and O3, the evolution characteristics, and the changing rules in different functional areas were analyzed. The results show that the concentration of atmospheric pollutants had obvious spatial-temporal evolution characteristics. The pollutants during the heat supply period were mainly affected by the coal-fired heating, and the major pollutants were particulate matters. The pollution situation in the industrial area was more serious comparing with those in other functional areas.

Due to the uneven distribution of water resources in Northeast China and the rapid development of urbanization, the natural ecological environment in Northeast China has been destroyed day by day, and the ecological environment of urban residential areas is deteriorating day by day. Based on the above situation, this paper puts forward the application of sponge city technology to the construction of urban district, from pavement, residential ecological park, underground parking lot to improve and innovate the situation of urban residential area in Northeast China.

(Ba_1-xHo_x)(Ti_1-xEr_x)O₃ ceramics (abbreviated BHTE) were prepared using the mixed-oxides route. The crystal structure, solid solubility, and defect chemistry of BHTE were investigated using X-ray diffraction (XRD), electron paramagnetic resonance (EPR) and photoluminescence. The results indicated that the solubility limit of Ho³⁺/Er³⁺ ions in the BaTiO₃ lattice is x = 0.01 and BHTE ceramics has a tetragonal perovskite structure. When x is more than 0.02, a small amount of Er₂O₃ separates out in BHTE, accompanied by Ba₆Ho₂Ti₄O₁₇ phase. The site occupation, solid solution formation, and defect chemistry are discussed.

In order to alleviate the increasing shortage of excellent mountain sand resources, method which sand mixed limestone powder instead of excellent mountain with natrual medi-sand is used, at the same time, taking break stone replace of pebble, and making small break stone and big break stone at the ratio of 3:7 to fabricate the C35～C60 pumping concrete for bridge with high properties. Combing with the example of Wu Song Bridge, C35～C60 pumping concrete with high strength and high properties application technology mixed by break stone and limestone powder double-doping technology was studied. By the optimization design of bridge-like concrete mix proportion, C35～C60 pumping concrete was mixed. Providing a route for high strength pumping concrete mixed by using Jinlin resources.

In order to overcome the problem that steel slag composite cement can only produce low grade cement and recycle waste of resources, the proportion, hydration mechanism and physical properties of the steel slag &granite composite cement by using different dosage of steel slag and granite. The results show that 42.5R composite cement can be obtained successfully by using steel slag 35%, clinker 35% and granite 25%.

A low cost polyacrylate superabsorbent hydrogel (SAP) was synthesized by aqueous solution polymerization. Its absorbency and salt-tolerance were studied subsequently. The results showed that the resin had a high water absorption rate of 220 g/g and 36g/gin water and salt solution, respectively. Then the hydrogel was suffered 60 ℃ high temperature to characterize the water retention. It showed that the resin have the ability to sustain water more than 720 minutes at high temperature. After repeated adsorption and discharge for five times, the adsorption amount can still reach 95.55% and 90.12% of the first adsorption amount respectively, demonstrating a good recycling adsorption performance. The high water absorbency was due to the synergistic effect of hydrophilic groups in the resin and osmotic pressure in the mesh. The presence of ions is the main reason for the decrease of water absorption ratio, but it still showed a high water absorption property, indicating that polyacrylic resin has a certain salt tolerance. The adsorption kinetics and diffusion kinetics of the resin in water and salt solution were studied. The diffusion kinetics showed that the polyacrylic acid in water was non-fickian diffusion, which was the main reason for the fast adsorption rate. Fickian diffusion was consistent in 0.9wt%NaCl solution, that is, metal ions played a leading role in the diffusion of polymer. The hydrogels with high water absorption and good water retention properties are expected to be used as water retention agents in agriculture, gardens and other fields.

In this paper, bisphenol-a poly(aryl ether ketone)s containing methyl and trifluoromethyl were synthesized respectively, and transparent polymer films were obtained by the sol-gel method. The structures of two poly(aryl ether ketone)s were determined by 1H-NMR. DSC study showed that the Tg of the bisphenol-a poly(aryl ether ketone)s with main chain containing methyl (BPA-PAEKs) was 159oC, and that the Tg of poly(aryl ether ketone)s with main chain containing trifluoromethyl (6FBPA-PAEKs) was 166oC, and the 5% weight loss of both polymers was more than 460oC, showing good thermal stability. Through the study on the contact Angle, it was found that the water contact Angle of the BPA-PAEKs was 78o, and that the water contact Angle of the 6FBPA-PAEKs was 91^o, the latter shows better hydrophobic performance.

Excessive iron content in human body can cause human tissue damage, skin pigmentation, liver and spleen dysfunction and other symptoms. In this paper, the reaction of Fe³⁺ with o-vanillin ethanol solution to form the dark blue complex was studied. O-vanillin ethanol solution has strong selectivity to Fe³⁺. In the range of Fe³⁺ concentration 0～200 μM , the ultraviolet light absorption of Fe³⁺ with different concentrations showed a good linear relationship with Fe³⁺ concentration (R=0.99299), the detection limit of o-vanillin ethanol solution on Fe³⁺ was about 1 M.

Nitrite wildly exists in natural environment, with excess nitrite that may cause methemoglobin or Blue baby syndrome. This study achieves a one-pot hydrothermal synthesis of polyethyleneimine-capped copper nanoclusters which based on CuSO₄ and PEI for detection of nitrite. Base on their formation of ground-state complex and fluorescence quenching. The limit of detection was 2 μM in the linear range of 0~1000 μM, which can detect the trace of nitrite.

Silver in modern life, such as medicine, drinking water disinfection has a wide range of applications, a large number of silver released into the natural environment, the microorganisms in the fish in the water and environmental damage based on the literature using the method of thermal water with bovine serum albumin as reductant synthesis of bovine serum albumin protect the shower of gold nanoparticles as probes, set up a colorimetric sensors used for rapid detection of silver ions This fluorescence probe has a good selectivity toward silver ions and a good linear relationship between 0.1 and 10 nM(R2=0.98746). A detection limit of sliver ions is 0.1 mM.

This paper introduced the synthesis and characterization of side chain amino-modified silicone oil. Side chain amino-modified silicone oil was prepared by Hexamethyldisiloxane (MM), octamethycyclotetrasiloxane (D4) and N,β-aminoethy-γ-aminopropylmethy dimethoxysilane (KH-602) through ring-opening polymerization. Through the analysis of viscosity, yield, and parallel experiments, the optimal dosage of MM, KH-602 and tetramethylammonium hydroxide (equivalent ratio) were obtained during the process of experiment. Furthermore, the structure and properties of side chain amino-modified silicone oil was characterized by various analytical techniques, including Fourier transform infrared spectroscopy (FT-IR), viscosity meter, and thermogravimetric analyzer. According to the analytical test results, the side chain amino-modified silicone oil possess excellent thermal stability and has crucial potential application value in the production and preparation of carbon fiber oil.

The common feature selection methods utilize the entire region of the sample space to extract an optimal subset of features. In contrast, this paper proposes a new local feature selection method, in which each region of the sample space is associated with a different optimal feature set, which can optimally adapt to the local variation of the sample space. At the same time, when solving the subspace corresponding to the optimal feature set, based on the concept of the nearest neighbor, this paper proposes a method to measure the similarity between the test data and each class to classify the test samples. The method proposed in this paper can be expressed as a linear programming optimization problem, so the global optimal solution can be solved by simple convex optimization. The experimental results on three real datasets and three mainstream methods demonstrate the feasibility and effectiveness of the proposed algorithm.