Photodynamic Therapy (PDT) is a new treatment that is different from surgery, radiotherapy, chemotherapy and immunotherapy. It has a good effect on the selective treatment of angiogenic tissues. This article compared the biocompatibility and photodynamic efficacy of three porphyrins and porphyrin-based polymers. MTT test results showed that Por, Por-PNIPAM, and Por-PNIPAM-b-POEGMA were less toxic to mouse fibroblasts L929 and human cervical cancer cells HeLa under non-light; while Por, Por-PNIPAM and Por-PNIPAM-b-POEGMA are less toxic to L929 cells, but higher to HeLa cells.