This paper expounds the significance of the Party building culture construction in colleges and universities for the work of organizing and educating people, and analyzes the relationship between the Party building culture and organizational education. Under the new situation of comprehensively and strictly administering the party, and combined with the reality of colleges and universities, this paper puts forward a new path for the construction of Party building culture in colleges and universities from three aspects: “strengthening the construction of inner-Party political culture in colleges and universities, carrying out the study and education of Party history, and innovating the construction of Party building culture carrier”. It also expounds the specific ideas of constructing “the collaborative mechanism between Party building culture and organizational education”.