The increase of communication interference signals has seriously affected the normal communication of multi devices in the Internet of things. Therefore, an independent component analysis based method for interference suppression of multi devices in the Internet of things is proposed. The interference signal of the multi equipment communication system of the Internet of things is transformed into comb jamming signal through comb filter and power amplifier. The independent component analysis of the interference signal is realized through the mixed model, Unmixing Model and assumption conditions of the independent component analysis, and the frequency hopping signal and comb jamming signal of the multi equipment of the Internet of things are separated to achieve interference suppression. The experimental results show that this method has good separation effect on mixed signals and can effectively suppress comb blocking interference in multi device communication of the Internet of things.