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Study on the chemical constituents and antioxidant activity of black tea from Hipopphae rhamnoides L. leaves in Jilin Province
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RAN Limei,ZHOU Hongli*,LIU Yuqi,LIU Ying
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Abstract
The chemical constituents and antioxidant potentials of Hipopphae rhamnoides L. leaves and its black tea product were investigated. Methods: The contents of tea polyphenols, flavonoids, polysaccharides and caffeine were analyzed by Folin colorimetric method, Sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method, Phenol-sulfuric acid method and national standard method of "Theophylline determination"; Four methods including DPPH?, ABTS+, ?OH and reducing power were used to evaluated antioxidant activity. Results: The main chemical components of the black tea: tea polyphenols was 18.45%, flavonoids content was 2.51%, polysaccharides content was 15.56% and caffeine content was 3.06%; the results of in vitro antioxidant experiments showed that the IC50 of Hipopphae rhamnoides L. leaves and its black tea on scavenging DPPH?, ABTS+ and?OH free radicals were 2.47 mg/mL and 0.76 mg/mL, 0.0040 mg/mL and 0.0038 mg/mL, 0.48 mg/mL and 0.41 mg/mL, respectively. They also had a certain reducing power and a good concentration dependence. Conclusions: The antioxidant activity of black tea is better because of active ingredients such as tea polyphenols, flavonoids and polysaccharides.
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Published: 25 September 2021
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