CdTe quantum dots were synthesized by hydrothermal method, and a method for quantitative analysis of clenbuterol hydrochloride was founded based on the interaction between clenbuterol hydrochloride and CdTe quantum dots. Results shown that clenbuterol hydrochloride had a fluorescence enhancement effect on the fluorescence emission intensity of CdTe quantum dots, and there was a good linear relationship between the increasing of fluorescence intensity (F/F₀) and the concentration of clenbuterol hydrochloride, the linear equations was F/F₀=0.3970C_clenbuterol+1.024, the detection of limit was 0.3402 nmol·L^-1. This method maybe provide an experimental basis for the quantitative analysis of clenbuterol hydrochloride by fluorescence method.