Rural domestic sewage treatment in northern of China is seriously affected by low temperature. In order to solve this problem, the activated sludge with good treatment effect on sewage at 8℃ was obtained by low temperature domestication, and its sewage treatment effect was analyzed. The results showed that when the domesticated sludge with dry weight concentration of 3.4g?L-1 was used to treat rural domestic sewage, the highest COD removal rate of the rural domestic sewage was 84.97%, the lowest COD of the effluent was 89.51 mg?L-1, and the shortest treatment time of reaching irrigation water quality standard of farmland (GB 5084-2005) was 8 days. Compared with the non-domesticated sludge, the COD removal rate of sewage after the activated sludge acclimated increased by 23.14%, which provided good technical support for rural domestic sewage treatment in northern of China.