Hydride generation-atomic fluorescence spectrometry (HG-AFS) was used to determine the content of selenium in Ligusticum chuanxiong. The sample of Ligusticum chuanxiong is digested by nitric acid, and the content of selenium is determined by HG-AFS with potassium hydroxide and potassium borohydride used as reducing agents. The analysis method of the hydride - atomic fluorescence spectrometry determination of selenium in Ligusticum chuanxiong was established. Under the optimum working conditions, the selenium contens express a good linear relationship in the range of 0-15 ng/mL. The standad curve equation is y=0.78x+31.35 with the correlation coefficient is 0.9902 and the detection limit is 0.96 ng/g.The selenium content in Ligusticum chuanxiong is 1.354 ng/g. This study can provide a scientific information for the determination of selenium and the evaluation of Ligusticum chuanxiong.