Fermentation engineering experiment is the core experimental course of biopharmaceutical major, which is based on many theoretical courses. This paper takes the experimental teaching of biopharmaceutical specialty of Jilin Institute of Chemical Technology as the subject, and plans to carry out the reform and exploration of the teaching content, methods and assessment of fermentation engineering experiment. The results show that: teaching content is optimized, the applied curriculum system is established effectively, the teaching mode is adjusted and diversified teaching modes are established, the assessment mode is reformed effectively, and the students' comprehensive quality is evaluated objectively. The practice of fermentation engineering experimental teaching reform fits the teaching characteristics of the cradle of engineer's growth.