With the continuous development of Internet technology, the potential security risks and attack threats in the network are more diversified and complicated. In this paper, the development trend of cyberspace security and the deficiency of practical teaching of network security attack and defense in colleges and universities are mainly studied, the design and research of college students’ attack and defense practice teaching platform based on cyberspace security is put forward combined with the actual situation, and the realization method, technology and function of the platform are discussed in detail, so as to apply this platform to students’ practical teaching and training, which helps improve students’ interest in learning cyberspace security and enhance students’ practical application ability of network security.