A strain of high efficiency flocculating to improve the removal of copper ions was selected from activated sludge of Jilin sewage treatment plant, and was amplified and identified by 16 S rDNA. The flocculating conditions of copper ion (0.2 ppm) by bioflocculant were studied. The results show that the flocculation efficiency is over 80%. Significant influencing factors and variables were pH, flocculation time and Graphene Oxide (GO) inducer which had major impact effects on flocculation efficiency. The highest flocculation efficiency which 86.01% achieved at pH=5, at 1.62h and 13.11mg/L GO as inducer.