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| Oestradiol Interferes with The Cytotoxicity of Benzo[a]pyrene by Enhancing NGF-induced Cell Differentiation |
| AN Mengyuan1， XIA Yu1， LI Wenwen1， ZHANG Shixu2 ，MA Lili2， ZHOU Yuhan1， HAO Zihan1， XU Liang 1*#br#
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Abstract In this study, benzo[a]pyrene (BaP) was used as the polluted background in PC12 cell culture studies.To track changes in cell phenotypic, proliferation rate, ROS value, and cell survival rate, oestradiol and NGF were introduced. Using the ggplot2 program and macrotranscriptome sequencing, a number of analyses were conducted on the number of genes, changes in gene expression, and gene expression between pathways.The findings demonstrated that when exogenous Nerve Growth Factor (NGF) was present, oestradiol promoted cells' endogenous NGF secretion. It stopped the overexpression of the anti-apoptotic protein Bcl-2 by fortifying the MAPK signaling pathway. Cell differentiation was induced downstream of the MAPK signaling pathway.Because the findings of the transcriptome test indicated that the PTPRO protein and the E2Fs transcription factor family were highly expressed. We confirmed the appropriate mRNA levels and focused on E2F1 to control PTPRO protein-induced neuronal differentiation based on our previous research.Lastly, we demonstrated the cytotoxicity of BaP and oestradiol by simulating their denaturation of the PTPRO protein using computer molecular docking simulation technologies. Currently, there is a dearth of research on the mechanism of interference between PAHs and estrogen. In order to address PAHs in the environment, we believe that our study will generate fresh research ideas.
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Published: 20 December 2025
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