含油废水中筛选的两种石油降解菌的鉴定及降解效果

doi:10.16039/j.cnki.cn22-1249.2018.07.005

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摘要采用9项生理生化试验以及16S rDNA技术对从含油废水中筛选的2株菌L2、L3进行鉴定，并对L2、L3菌株降解石油烃效果进行了研究。结果表明：2种菌株分别为：L2—Ochrobactrum ciceri sp. 苍白杆菌和L3—Serratia marcescens sp.粘质沙雷氏菌。2株单菌对石油烃的降解效果非常高效，初始浓度为4000 mg/L的石油烃，分别加入菌株L2、L3，120 r/min、30℃下振荡培养6天后，总石油烃降解率分别高达97.37%、98.98%。两种菌在前3天对石油降解速率较快，随后进入稳定期生长速率平缓直至趋于零。

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	刘虹
	高鹏飞
	丛唯一
	王明超
	温钢
	田朕

关键词: 含油废水鉴定石油烃降解

Abstract: Two strains screened from oil wastewanter were identified using 9 physiological and biochemical tests,16s rDNA technology and degradation effect of petroleum hydrocarbons components by two single strain were studied. Results showed that the two strains were:L2-Ochrobactrum ciceri sp.and L3-Serratia marcescens sp. The effect of 2 strains of single bacteria on petroleum hydrocarbon degradation was very efficient,It revealed that petroleum hydrocarbons degradation rate of L4 was up to 97.37% and 98.98% after incubated in a rotary shaker at 30℃ and 120 r/min for 6 days and initial concentration of petroleum hydrocarbons was 4000 mg/L. Two kinds of bacteria on the first 3 days of the oil degradation rate was faster, and then enter the stationary phase, the growth rate was slow until zero.

Key words: Oily wastewater identification petroleum hydrocarbon degradation

出版日期: 2018-07-25 发布日期: 2018-07-25 整期出版日期: 2018-07-25

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引用本文:

刘虹, 高鹏飞, 丛唯一, 王明超, 温钢, 田朕. 含油废水中筛选的两种石油降解菌的鉴定及降解效果[J]. 吉林化工学院学报, 2018, 35(7): 21-25.
LIU Hong, GAO Peng fei, CONG Weiyi, Wang Mingchao, Wen Gang, Tian Zhen. Identification and degradation effect of two oil degrading bacteria screened from oily wastewater. Journal of Jilin Institute of Chemical Technology, 2018, 35(7): 21-25.

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https://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2018.07.005 或 https://xuebao.jlict.edu.cn/CN/Y2018/V35/I7/21

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