Our country pays more attention to the cultivation of talents in colleges and universities under the background of the strategic deployment of education modernization. To meet the demand of scientific research and innovation ability of universities, new instruments and equipment are constantly introduced into university laboratories. However, due to the lack of full-time laboratory personnel, management system and safety concept, teachers and students do not have a high understanding of the importance of laboratory safety, which increases the risk of laboratory accidents in colleges and universities. Therefore, it is particularly important to effectively analyze the cause and explore the emergency treatment of unsafe behavior in university laboratories.