Exploring the integrity culture construction plays the guiding role in the construction of teachers' ethics in the new era. By explaining the significance of the new era to strengthening integrity culture in colleges and universities, this paper analyzes the connotation and value of integrity culture, explores the intrinsic connection between integrity culture construction and the development of college teachers' ethics and conduct, analyze the new era present situation of the construction of college ethics strengthen and problems, and examines the current situation and issues related to teachers' ethics and conduct in higher education in the new era. In the end, it puts forward new thoughts and approaches for enhancing the construction in colleges and universities by taking integrity culture construction as a guiding principle.