The silver-nano solution was synthesized by reacting silver nitrate with ulla grass and employed to modify linen and wool fabrics. The modified fabrics underwent SEM, FTIR, UV-Vis analysis, as well as antibacterial and washability testing. Optimal modification conditions for achieving bacteriostatic effects on E. coli and B. subtilis were determined by measuring the diameter of the bacteriostatic zone. Results indicated that both modified linen and wool fabrics exhibited a significant presence of particles on their surfaces, with antibacterial bands against E. coli and B. subtilis exceeding 1 mm. Furthermore, the antibacterial rate remained above 95% even after 20 washing cycles. Overall, the treatment process involving reduction of silver nanoparticles led to uniform attachment onto wool fabrics, thereby enhancing their antibacterial properties without compromising original performance characteristics. This environmentally friendly production method offers simplicity in operation while minimizing industrial waste water discharge.