An analytical method for the determination of antioxidant 3052 by HPLC. The determination conditions were as follows: C18 column (WondaSil 4.6 mm×250 mm, 5 μm), The mobile phase consisted of methanol and deionized water (95:5, v/v),  UV detector at 208 nm, the flow rate was 1 mL/min and the injection volume was 20 μL. The result shows that the mass concentration was 0.015 ~ 0.21 mg/mL, the linear regression equation was y=49989x+33.976(r2=0.9998) and the recovery rated of the sample was 98.1%~101.5%.