Fluorescence and absorbance spectra was obtained to study the interaction between Ponceau 4R/Amaranth and graphene/CdTe quantum dots composites. Both Ponceau 4R/Amaranth have strong fluorescence quenching effect on graphene/CdTe quantum dots composites, and both were static quenching process. But quenching rate caused by ponceau 4R/amaranth was different, because of the difference of structure. Based on variable temperature experiment、absorbance spectrum and structure analysis, we could conclude that conjugated degree of ponceau 4R is higher than that of amaranth. Furthermore there was a good linear relationship between ponceau 4R/amaranth and graphene/CdTe quantum dots composites, and could be used for quantitative analysis of ponceau 4R/amaranth.