一株降解石油烃假单胞菌的鉴定及降解特性

doi:10.16039/j.cnki.cn22-1249.2020.03.005

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摘要

从松原油田石油污染土壤中筛选、分离出一株降解石油烃的菌株，观察其菌落形态，通过16S rDNA 序列分析对其进行鉴定。结果表明：该菌株的菌落呈白色不规则状、表面粗糙、边缘不整齐。16S rDNA序列分析鉴定为类产碱假单胞菌（Pseudomonas pseudoalcaligenes strain）。在温度为35℃、pH值为8时菌株对石油烃降解效果最好，降解率为75.4%。

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	刘虹
	曾繁城
	高鹏飞

关键词: 石油烃假单胞菌鉴定降解效果

Abstract:

A strain that degrades petroleum hydrocarbon was screened and isolated from oil-contaminated soil of Songyuan oilfield, its colony morphology was observed, and it was identified by 16S rDNA sequence analysis. The results indicated that the colony was white and irregular with rough surface and irregular edge. The 16S rDNA sequence was identified as Pseudomonas pseudoalcaligenes strain. When the temperature was 35℃ and the pH value was 8, the strain had the best degradation effect on petroleum hydrocarbon, and the degradation rate was 75.4%.

Key words: petroleum hydrocarbon pseudomonas identification degradation effect

出版日期: 2020-03-25 发布日期: 2020-03-25 整期出版日期: 2020-03-25

ZTFLH:

X 592

基金资助:

吉林市科技发展计划资助项目（201831715）

引用本文:

刘虹, 曾繁城, 高鹏飞. 一株降解石油烃假单胞菌的鉴定及降解特性 [J]. 吉林化工学院学报, 2020, 37(3): 24-27.
LIU Hong, ZENG Fancheng, GAO Pengfei. Identification and Degradation Characteristics of a Strain of Pseudomonas . Journal of Jilin Institute of Chemical Technology, 2020, 37(3): 24-27.

链接本文:

http://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2020.03.005 或 http://xuebao.jlict.edu.cn/CN/Y2020/V37/I3/24

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[2]	董瑶, 钱元梅, 宋曼, 常迪, 崔浩. *利用线粒体DNA上的cytB鉴定林蛙及林蛙油* [J]. 吉林化工学院学报, 2019, 36(1): 52-57.
[3]	刘虹, 高鹏飞, 丛唯一, 王明超, 温钢, 田朕. 含油废水中筛选的两种石油降解菌的鉴定及降解效果[J]. 吉林化工学院学报, 2018, 35(7): 21-25.

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