姜黄素通过调节p38 MAPK通路抑制前脂肪细胞3T3-F442A成脂分化

doi:10.16039/j.cnki.cn22-1249.2024.07.011

下载:

PDF (3567KB)
输出: BibTeX | EndNote (RIS)

摘要 目的：研究姜黄素对前脂肪细胞3T3-F442A分化和脂肪生成的影响。方法：采用MTT法检测0~100 μM的姜黄素在24、48、72 h对3T3-F442A细胞增殖的影响；使用添加30 μM姜黄素的诱导分化培养基处理前脂肪细胞3T3-F442A，通过油红O染色法观察姜黄素对前脂肪细胞3T3-F442A脂肪生成的影响；采用RT-qPCR技术、Western Blot实验、ELISA实验检测姜黄素对前脂肪细胞3T3-F442A分化相关转录因子、脂肪生成相关血管内皮生长因子及受体以及基质金属蛋白酶表达的影响、p38 MAPK的磷酸化水平的影响。结果：姜黄素能抑制3T3-F442A脂肪细胞增殖，下调脂肪细胞分化相关转录因子、血管内皮生长因子及受体以及基质金属蛋白酶的表达水平，还下调了p38 MAPK的磷酸化水平。结论：姜黄素可以通过抑制p38 MAPK信号通路以及降低基质金属蛋白酶的活性，从而抑制前脂肪细胞3T3-F442A的分化和脂肪生成。

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	（）
	作者相关文章
	程亚青
	刘爱萍
	李佟
	张仁文
	葛雅琨
	张元新

关键词: 姜黄素脂肪细胞细胞分化脂肪生成转录因子基质金属蛋白酶

Abstract: Objective: The effects of curcumin on the differentiation and adipogenesis of 3T3-F442A preadipocytes are investigated. Methods: The effects of 0-100 μM curcumin on the proliferation of 3T3-F442A cells at 24, 48 and 72 h are detected by MTT assay; 3T3-F442A preadipocytes are treated with induced differentiation medium supplemented with 30 μM curcumin, and the effects of curcumin on the adipogenicity of 3T3-F442A preadipocytes are observed by Oil Red O staining method; RT-q PCR, Western blot, and ELISA assay are used to detect the effects of curcumin on the expression of 3T3-F442A preadipocyte differentiation-related transcription factors, adipogenesis-associated vascular endothelial growth factors and receptors, and matrix metalloproteinases expression, phosphorylation levels of p38 MAPK. Results: Curcumin inhibits the proliferation of 3T3-F442A adipocytes, down-regulated the expression levels of adipocyte differentiation-related transcription factors, vascular endothelial growth factors and receptors, and matrix metalloproteinases, and also down-regulates the phosphorylation level of p38 MAPK. Conclusion: Curcumin inhibits 3T3-F442A preadipocyte differentiation and adipogenesis by inhibiting the p38 MAPK signalling pathway and matrix metalloproteinase activity.

Key words: curcumin adipocytes cell differentiation adipogenesis transcription factors matrix metalloproteinase

出版日期: 2024-07-25 发布日期: 2024-07-25 整期出版日期: 2024-07-25

R285.5

引用本文:

程亚青, 刘爱萍, 李佟, 张仁文, 葛雅琨, 张元新. 姜黄素通过调节p38 MAPK通路抑制前脂肪细胞3T3-F442A成脂分化[J]. 吉林化工学院学报, 2024, 41(7): 65-71.
CHENG Yaqing, LIU Aiping, LI Tong, ZHANG Renwen, GE Yakun, ZHANG Yuanxin. Curcumin Inhibits Lipogenic Differentiation of 3T3-F442A Preadipocytes by Modulating the p38 MAPK Pathway. Journal of Jilin Institute of Chemical Technology, 2024, 41(7): 65-71.

链接本文:

https://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2024.07.011 或 https://xuebao.jlict.edu.cn/CN/Y2024/V41/I7/65

[1]	. [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 0 .
[2]	. [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 0 .
[3]	SHAO Bao-li, LU Da, ZHAO Dong-hui. The Application of Dimensional Analysis in the Physical Quantity Conversion between Physical System and Numerical System [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 1 -3 .
[4]	ZHANG Jian, ZHAO Xiang, QU Bo, WU Qi, LIU Yu-tong, LI Yu-shi, LIU Qun. Application of Phosphorus-sulfur-nitrogen Composite Flame Retardant in Cotton Fabric [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 4 -7 .
[5]	WU Ping, REN Hong, LU Fei, WEI Qingling. A Functional Material on Recognition of Zn(II) ions based on the New Azo Compound [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 8 -10 .
[6]	YANG Yan-jun, WANG Ya-hong, Yang Xiu-dong. Process Aptimization of Surfactant Assisted Extraction of Total Polyphenols from Kyllinga Brevifolia Rottb [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 11 -15 .
[7]	LIU Jin-lu, LEI Yong-ping, WANG xiao-lin, ZHONG fang-li. Study on the Purification Method of Total Saponins fromFruit of Rosa Davuvrica Pall. and its Purification Method [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 16 -23 .
[8]	SONG Jian-gang, ZHONG Fang-li, WANG Xiao-lin, LIN Yu. Study on Extraction of Anthocyanin from Aronia melanocarpa Fruit by Ionic liquid Ultrasound Assisted [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 24 -31 .
[9]	TAN Li-hui, TAN Hong-wu. The Crashworthiness Analysis of different Cross-Section Thin-Walled Components [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 32 -35 .
[10]	YU Wen-xin, ZHENG Kai, WANG Li-hui, LIU Hai-bo, Wang Jian-xin. The Influence of Magnetostrictive Transducer Radiation Plate material on Radiation Sound Field Distribution [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 36 -40 .