托盘根多糖初步纯化及其体外抗氧化活性研究

doi:10.16039/j.cnki.cn22-1249.2018.07.021

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摘要采用水提醇沉法提取托盘根多糖，粗多糖脱蛋白、透析后经DEAE-52纤维柱色谱分离纯化得到2个纯化多糖：RCP-1和RCP-2。通过体外清除DPPH、超氧阴离子和ABTS自由基评价纯化多糖的体外抗氧化活性。结果表明：RCP-1和RCP-2多糖含量分别为80.66%和82.15%。体外抗氧化实验表明，托盘根纯化多糖RCP-1和RCP-2在实验浓度范围内对DPPH、超氧阴离子和ABTS自由基均有一定的清除作用，同时具有良好的浓度依赖关系，其中RCP-2的自由基清除作用明显高于RCP-1。说明托盘根多糖具有较好的抗氧化活性，可能是其水溶性成分生物活性的物质基础。

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	杨秀东
	张嫔妹
	张艳
	张扬
	崔浩
	周鸿立

关键词: 托盘根多糖分离纯化抗氧化活性

Abstract: The crude polysaccharide of Rubus crategefolius Bunge. root was extract using water-extract and ethanol-precipitation method. It was further purified through de-protein and dialysis as well as DEAE-52 cellulose column chromatography. Two purified polysaccharide, RCP-1 and RCP-2 were obtained. The in vitro antioxidant activity of RCP-1 and RCP-2 was determined by DPPH, superoxide anion and ABTS scavenging assays. The results showed that the polysaccharide content of RCP-1 and RCP-2 were 80.66% and 82.15%, repectively. RCP-1 and PCP-2 had scavenging effect on DPPH, ABTS and superoxide anion. It also showed the concentration-dependent relationship. RCP-2 showed higher antioxidant activity that RCP-1. The results indicated that polysaccharide from Rubus crategefolius Bunge. root have a good antioxidant activity. It might be the material basis of the bioactivity of water soluble component of Rubus crategefolius Bunge. root.

Key words: Root of Rubus crategefolius Bunge Polysaccharide Isolation and Purification Antioxidant activity

出版日期: 2018-07-25 发布日期: 2018-07-25 整期出版日期: 2018-07-25

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引用本文:

杨秀东, 张嫔妹, 张艳, 张扬, 崔浩, 周鸿立. 托盘根多糖初步纯化及其体外抗氧化活性研究[J]. 吉林化工学院学报, 2018, 35(7): 90-94.
YANG Xiu-dong, ZHANG Pin-mei, ZHANG Yan, ZHANG Yang, CUI Hao, ZHOU Hong-li. The Preliminary Purification of Polysaccharide from Rubus crategefolius Bunge. Root and Their Antioxidant Activity in vitro. Journal of Jilin Institute of Chemical Technology, 2018, 35(7): 90-94.

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https://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2018.07.021 或 https://xuebao.jlict.edu.cn/CN/Y2018/V35/I7/90

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