全二维气相色谱 / 飞行时间质谱对微生物降解石油烃产物的分析

doi:10.16039/j.cnki.cn22-1249.2022.01.003

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摘要

采用气相色谱对一株高效石油烃降解菌株微嗜酸寡养单胞菌(Stenotrophomonas acidaminiphila)降解石油烃的产物进行分析，其中８０％以上的石油烃被降解，在产物色谱图中，出现了ＵＣＭ峰，因而，进一步采用全二维气相色谱/ 飞行时间质谱分析了菌株降解石油烃的产物.结果表明:该菌株能够降解大部分Ｃ１０-Ｃ２８正构烷烃、部分直链烷烃和环烷烃，芳香烃尤其是多环芳烃很难被该菌株降解.由检测到中间产物推断:直链烷烃如十六烷，在微生物作用下，生成中间产物十六烷醇，接着被氧化为十六醛和脂肪酸，脂肪酸被进一步氧化，最终生成ＣＯ２和Ｈ２Ｏ支链烷烃的降解途径和直链烷烃相似?检测到中间产物２-己基-１-十二醇、２-辛基-１-癸醇、２-甲基-１-辛醇等，环烷烃先被氧化为环醇，接着形成环酮，然后被进一步降解，检测到的中间产物有１-环己醇、环辛烷甲醇、３-甲基环戊醇、环庚烷甲醇等，烷基芳香烃被氧化成羧酸，芳香烃苯环羟被基化，随后进行芳环裂解。

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	刘虹１
	曾繁城１
	高鹏飞２
	黄毅３
	赵炳岩１
	刘子铭１

关键词: 全二维气相色谱飞行时间质谱微生物石油烃降解产物

Abstract:

Gas chromatography was used to analyze the petroleum hydrocarbon degradation products of a highly efficient petroleum hydrocarbon degrading strain Stenotrophomonas acidaminiphila. More than 80% of petroleum hydrocarbons were degraded.UCM peak appeared in the product chromatogram.The results indicated that the strain was able to degrade most of the C10-C28 n-alkanes,some straight-chain alkanes and cycloalkanes, while the aromatic hydrocarbons,especially polycyclic aromatic hydrocarbons,were difficult to degrade by the strain According to the intermediate products detected,straight-chain alkanes,such as hexadecane,under the action of microorganisms,generate intermediate products hexadecanol,which was then oxidized to hexadecaldehyde and fatty acids.Fatty acids are further oxidized to finally produce CO, and H,O.The degradation pathway of branched-chain alkanes is similar to that of straight-chain alkanes,and the intermediate products detected were2-hexyl-1-dodecyl alcohol,2-octyl-1-decan

Key words: comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry microbes petroleum hydrocarbon degradation products

出版日期: 2022-01-25 发布日期: 2022-01-25 整期出版日期: 2022-01-25

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引用本文:

刘虹１, 曾繁城１, 高鹏飞２, 黄毅３, 赵炳岩１, 刘子铭１. 全二维气相色谱 / 飞行时间质谱对微生物降解石油烃产物的分析 [J]. 吉林化工学院学报, 2022, 39(1): 15-20.
LIU Hong, ZENG Fancheng, GAO Pengfei, HUANG Yi, ZHAO Bingyan, LIU Ziming. Analysis of Microbial Degradation Products of Petroleum Hydrocarbon by Comprehensive Two -Dimensional Gas Chromatography Coupled toTime-of-Flight Mass Spectrometry . Journal of Jilin Institute of Chemical Technology, 2022, 39(1): 15-20.

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https://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2022.01.003 或 https://xuebao.jlict.edu.cn/CN/Y2022/V39/I1/15

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