响应面法优化微波萃取血红铆钉菇多糖及抗氧化活性研究

doi:10.16039/j.cnki.cn22-1249.2021.01.002

摘要
相关文章
推荐阅读
Metrics

下载:

PDF (4955KB)
输出: BibTeX | EndNote (RIS)

摘要

本文通过微波萃取技术优化血红铆钉菇多糖提取的最佳工艺，并研究其抗氧化活性。具体采用响应面分析法优化微波萃取血红铆钉菇多糖的提取工艺、Sevage法去除血红铆钉菇粗多糖中的蛋白质、DPPH法评价血红铆钉菇多糖的体外抗氧化活性。选取液料比、微波功率、微波温度和微波时间为响应面分析法的4个工艺参数因素，分析其对多糖得率的影响，采用 Design-Expert 软件对试验数据进行二次响应面分析。最终得到最优提取工艺条件为：微波时间15.33 min、微波温度88.94℃、微波功率621.49 W、液料比53.22: 1 (mL/g)，此时多糖得率可达到15.01%。经过6次Sevage法去除血红铆钉菇粗多糖中的蛋白质，蛋白质去除率可达到97%。纯化后的血红铆钉菇多糖对DPPH自由基的清除率达到最高77%。结果显示，微波萃取技术提取血红铆钉菇多糖技术可行，所提多糖具有较好的抗氧化活性。

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	（）
	作者相关文章
	吴也
	刘中正
	宋见喜
	姜贵全

关键词: 血红铆钉菇多糖微波萃取响应面抗氧化

Abstract:

To optimize the condition of microwave extracting Chroogomphidius viscidus polysaccharides (CVP) and evaluate the antioxidant activity of CVP. Response surface methodology was used to optimize the extraction process of polysaccharides extracted from Chroogomphidius viscidus, sevage was used to remove proteins from the crude polysaccharides of Chroogomphidius viscidus, and DPPH was used to evaluate the antioxidant activity of CVP in vitro. Selecting the ratio of liquid to material, microwave power, microwave temperature and microwave time as the four parameters of the response surface analysis method, and analyzing its influence on the yield of polysaccharides, using the Design-Expert software for the second response surface analysis of the test data. The optimum extraction process conditions were as follows: microwave time 25 min, microwave temperature 70°C, microwave power 780 W, liquid-to-feed ratio 30:1, and polysaccharide yield reached 15.00%. After 6 times sevage removal of protein in the crude polysaccharide of Chroogomphidius viscidus, the protein removal rate can reach 97%. The highest scavenging rate of the purified CVP on DPPH free radical can reach 77%. Microwave extraction technology is suitable in the extraction of CVP, and CVP shows a stronger antioxidant capacity.

Key words: Chroogomphidius viscidus Polysaccharide Microwave extraction Response surface:Antioxidant

出版日期: 2021-01-15 发布日期: 2021-01-15 整期出版日期: 2021-01-15

R284.2

引用本文:

吴也, 刘中正, 宋见喜, 姜贵全. 响应面法优化微波萃取血红铆钉菇多糖及抗氧化活性研究 [J]. 吉林化工学院学报, 2021, 38(1): 4-12.
WU Ye, LIU Zhongzheng, SONG Jianxi, JIANG Guiquan. Optimization of the Microwave Extraction Condition and Antioxidant Activity of Polysaccharides from Chroogomphidius Viscidus Using Response Surface Analysis . Journal of Jilin Institute of Chemical Technology, 2021, 38(1): 4-12.

链接本文:

https://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2021.01.002 或 https://xuebao.jlict.edu.cn/CN/Y2021/V38/I1/4

[1]	胡鹏, 毕慕涵, 周双萍, 辛蕊, 周鸿立, 王慧竹. 灵芝芽化学成分分析的研究 [J]. 吉林化工学院学报, 2024, 41(7): 7-11.
[2]	刘艳杰, 丁博文, 李瑞端, 王犇. 工业废液正丙醇回收工艺模拟与优化 [J]. 吉林化工学院学报, 2023, 40(3): 11-16.
[3]	杨艳俊, 杨秀东, 张艳. 山里红总黄酮超声提取及抗氧化活性研究 [J]. 吉林化工学院学报, 2022, 39(1): 1-9.
[4]	尹佳, 王玉瑶, 谢富忠, 刘得鹏, 李继锋. 葡萄籽提取物对柑橘果实的保鲜效果研究 [J]. 吉林化工学院学报, 2021, 38(9): 97-101.
[5]	冉丽梅, 周鸿立, 刘宇琦, 刘莹. 吉林地区沙棘红茶活性成分及抗氧化活性的研究 [J]. 吉林化工学院学报, 2021, 38(9): 5-8.
[6]	王喜印, 许诺, 李齐, 于东林. 基于响应面分析法的辊子式榛子破壳机结构参数的优化 [J]. 吉林化工学院学报, 2021, 38(9): 73-77.
[7]	王昭国. 基于Box-Behnken Design优化当归中铅元素的测定方法研究 [J]. 吉林化工学院学报, 2021, 38(3): 7-12.
[8]	劳军, 王楠楠. 不同浓度黄芪多糖在SH-SY5Y细胞内外抗氧化的研究 [J]. 吉林化工学院学报, 2020, 37(9): 77-80.
[9]	杨艳俊, 杨秀东, 王亚红. 景天三七总酚酸的提取工艺及抗氧化性研究 [J]. 吉林化工学院学报, 2020, 37(3): 33-37.
[10]	李婷婷, 薛天福, 宋长虹, 杨梅. 优化补料同步糖化发酵玉米穰产燃料乙醇的研究 [J]. 吉林化工学院学报, 2019, 36(7): 72-75.
[11]	赵鹤鹏, 刘佳南. 响应面法优化微波辅助提取沙棘籽油的工艺研究 [J]. 吉林化工学院学报, 2019, 36(11): 17-21.
[12]	牟莹莹, 夏一菲, 任婷, 王贺, 宋文刚. 升麻多糖的提取及其体外抗氧化活性研究 [J]. 吉林化工学院学报, 2019, 36(1): 94-96.
[13]	李卉, 张艳. 人参皂苷与远志皂苷配伍治疗大鼠阿尔茨海默病的作用研究[J]. 吉林化工学院学报, 2018, 35(7): 66-70.
[14]	杨秀东, 张嫔妹, 张艳, 张扬, 崔浩, 周鸿立. 托盘根多糖初步纯化及其体外抗氧化活性研究[J]. 吉林化工学院学报, 2018, 35(7): 90-94.
[15]	高瑀婷, 戴传波. 聚酚抗氧化剂CPL改性研究[J]. 吉林化工学院学报, 2018, 35(7): 26-28.

[1]	LIU Hong-bo, LIU Qi. Experimental Study on Pneumatic Space Bending Flexible Joint[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(3): 36 -39 .
[2]	ZHANG Yun-peng. Preparation and Characterization of Er³⁺，Y³⁺，Yb³⁺ Co-doped Al₂O₃ Luminescence Materials[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(3): 58 -63 .
[3]	XU You-zhuan, ZHOU Hou-qing. The Lower Bounds of Energy for Some Circulant Graph[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(3): 67 -72 .
[4]	ZENG Xiao-dan, JIANG Cheng, XI Hao, ZHU Lin. The Study of Determination of Carbon Tetrachloride Divergence in the Chlorosulfonated Polyethylene (CSM) Production Process[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(1): 8 -12 .
[5]	SHI Dai, GAO Song, LIN Guo-qing. Temperature Analysis of Anaerobic Fermentation Tank Based on ANSYS[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(1): 18 -22 .
[6]	WANG Yi-nuo, GU Feng, CONG Wei-yi, BAI Lu, WU Chun-ying. Luoma Lake Water Quality Variation Characteristics and Protection Countermeasures[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(1): 33 -36 .
[7]	ZHAO Ye-zhuo, LU Da-yong. Comparative Study on X-ray Diffraction of Several Vitamins[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(1): 37 -39 .
[8]	WANG Bing-xin. Research on Problems in Colleges’and Universities’Budget Management[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(2): 99 -102 .
[9]	YU Hong-chen. Analysis of the Regulating Valve Stem Fracture in Steam Turbine Feed Pump[J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(5): 54 -57 .
[10]	. [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(6): 0 .

Viewed

Full text

Abstract

Cited

Shared

Discussed