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吉林化工学院学报, 2020, 37(1): 13-16     https://doi.org/10.16039/j.cnki.cn22-1249.2020.01.003
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HPLC法同时测定牛黄清胃丸中四种成分的含量
于文萃1,高毓涛2*
1.辽宁地质工程职业学院,丹东 118000;2.丹东市市场监管事务服务中心,丹东 118002
Simultaneous Determination of Four Components in Niuhuangqingwei Pills by HPLC
YU Wencui1,GAO Mintao2*
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摘要 

建立同时测定牛黄清胃丸中大黄酸、大黄素、大黄酚和大黄素甲醚含量的HPLC法。方法:采用Diamonsil C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸(75:25),柱温为30 ℃,进样量为10μL,流速为1.0 mL·min-1,检测波长为254nm。结果:大黄酸、大黄素、大黄酚和大黄素甲醚的线性范围分别为22.68~113.40μg·mL-1(r=0.9991)、6.25~31.24μg·mL-1(r=0.9996)、14.74~73.68μg·mL-1(r=0.9990)和5.22~26.12μg·mL-1(r=0.9999),平均回收率分别为99.9%(RSD=1.2%,n=6)、99.9%(RSD=1.9 %,n=6)、100.0%(RSD=1.2%,n=6)和99.8%(RSD=1.8%,n=6)。结论:该方法简便、快速、准确,专属性强,样品处理简便,适用于牛黄清胃丸的质量控制。

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于文萃
高毓涛
关键词:  牛黄清胃丸  大黄酸  大黄素  大黄酚  大黄素甲醚  含量测定     
Abstract: 

To establish an HPLC method for the simultaneous determination of rhein, emodin, chrysophanol and physcion in Niuhuangqingwei pills. Methods: Diamonsil C18 (250 mm×4.6 mm, 5 μm) at 30 ℃ was used with methanol and 0.1% phosphoric acid (75: 25) as the mobile phase, the flow rate was 1.0 ml·min-1 and the detection wavelength was 254 nm. Results: The linear ranges of rhein, emodin, chrysophanol and physcion were at 22.68-113.40 μg·mL-1(r=0.9991), 6.25~31.24 μg·mL-1(r=0.9996), 14.74~73.68 μg·mL-1(r=0.9990) and 5.22~26.12 μg·mL-1(r=0.9999) respectively, the average recoveries (n=6) were 99.9%(RSD=1.2%), 99.9%(RSD=1.9%), 100.0%(RSD=1.2%) and 99.8%(RSD=1.8%).

Key words:  Niuhuangqingwei pills    rhein    emodin    chrysophanol    physcion    Content determination
               出版日期:  2020-01-25      发布日期:  2020-01-25      整期出版日期:  2020-01-25
R927.2  
引用本文:    
于文萃, 高毓涛. HPLC法同时测定牛黄清胃丸中四种成分的含量 [J]. 吉林化工学院学报, 2020, 37(1): 13-16.
YU Wencui, GAO Mintao. Simultaneous Determination of Four Components in Niuhuangqingwei Pills by HPLC . Journal of Jilin Institute of Chemical Technology, 2020, 37(1): 13-16.
链接本文:  
https://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2020.01.003  或          https://xuebao.jlict.edu.cn/CN/Y2020/V37/I1/13
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