The liver injury, with more etiology and complex mechanism, involves short-term sudden hepatocyte damage. Animal experiment is essential assay which used for researching the pathogenesis of the disease and drug screening on preclinical. The article is reviewed of liver injury based on the model which includes CCl₄, D-galactosamine, acetaminophen, ethanol and Chimeric mice with humanizedliver, which provides a targeted selection methods for animal experiments to research liver injury.