HPLC法同时测定牛黄清胃丸中四种成分的含量

doi:10.16039/j.cnki.cn22-1249.2020.01.003

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摘要

建立同时测定牛黄清胃丸中大黄酸、大黄素、大黄酚和大黄素甲醚含量的HPLC法。方法：采用Diamonsil C18色谱柱（250mm×4.6mm，5μm），流动相为甲醇-0.1%磷酸（75:25），柱温为30 ℃，进样量为10μL，流速为1.0 mL·min-1，检测波长为254nm。结果：大黄酸、大黄素、大黄酚和大黄素甲醚的线性范围分别为22.68～113.40μg·mL-1(r=0.9991)、6.25～31.24μg·mL-1(r=0.9996)、14.74～73.68μg·mL-1(r=0.9990)和5.22～26.12μg·mL-1(r=0.9999)，平均回收率分别为99.9%（RSD=1.2%，n=6）、99.9%（RSD=1.9 %，n=6）、100.0%（RSD=1.2%，n=6）和99.8%（RSD=1.8%，n=6）。结论：该方法简便、快速、准确，专属性强，样品处理简便，适用于牛黄清胃丸的质量控制。

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	于文萃
	高毓涛

关键词: 牛黄清胃丸大黄酸大黄素大黄酚大黄素甲醚含量测定

Abstract:

To establish an HPLC method for the simultaneous determination of rhein, emodin, chrysophanol and physcion in Niuhuangqingwei pills. Methods: Diamonsil C18 (250 mm×4.6 mm, 5 μm) at 30 ℃ was used with methanol and 0.1% phosphoric acid (75: 25) as the mobile phase, the flow rate was 1.0 ml·min-1 and the detection wavelength was 254 nm. Results: The linear ranges of rhein, emodin, chrysophanol and physcion were at 22.68-113.40 μg·mL-1(r=0.9991), 6.25～31.24 μg·mL-1(r=0.9996), 14.74～73.68 μg·mL-1(r=0.9990) and 5.22～26.12 μg·mL-1(r=0.9999) respectively, the average recoveries (n=6) were 99.9%（RSD=1.2%）, 99.9%（RSD=1.9%）, 100.0%（RSD=1.2%） and 99.8%（RSD=1.8%).

Key words: Niuhuangqingwei pills rhein emodin chrysophanol physcion Content determination

出版日期: 2020-01-25 发布日期: 2020-01-25 整期出版日期: 2020-01-25

ZTFLH:

R927.2

引用本文:

于文萃, 高毓涛. HPLC法同时测定牛黄清胃丸中四种成分的含量 [J]. 吉林化工学院学报, 2020, 37(1): 13-16.
YU Wencui, GAO Mintao. Simultaneous Determination of Four Components in Niuhuangqingwei Pills by HPLC . Journal of Jilin Institute of Chemical Technology, 2020, 37(1): 13-16.

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http://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2020.01.003 或 http://xuebao.jlict.edu.cn/CN/Y2020/V37/I1/13

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