Metabolomic Mechanism of Hepatotoxicity Induced by Oxidative Liver Injury Treated with High-dose Longdan Xiegan Decoction
ZHANG Ranran 1,
ZHANG Yan 2*, YANG Xiudong 1
(1.School of Chemical and Pharmaceutical Engineering, Jilin Insititute of Chemical Technology, Jilin City 132022,China; 2.School of Medicine, Jiaxing University, Jiaxing 314001,China)
Abstract: D-galactose was used to establish a rat model of oxidative liver injury, and to study the mechanism of Longdan Xiegan Decoction (LXD) in the treatment of oxidative liver injury. Rats were killed after 30 days of continuous administration, and biological samples such as liver tissue and blood were collected as planned. Gas chromatography-mass spectrometry was used to detect the related markers in liver homogenate and blood, and metabonomic was used to search the database, to analyze the differential metabolites of LXD in the treatment of oxidative liver injury in rats, and to analyze the metabolic pathways of the detected markers, finally, the mechanism of LXD in the treatment of oxidative liver injury in rats was determined. The experimental results show that, the biomarkers associated with the treatment of oxidative liver injury in rats were citric acid, 2-Aminobenzoic acid, hydroxylamine, α-ketoglutaric acid, cholesterol, sarcosine, nicotinic acid, nicotinamide, Pyridoxal Phosphate, itaconic acid, methyl phosphate, pyruvic acid, tyrosine, glyceraldehyde 3-phosphate, cisconite acid and oxalic acid. The mechanism of LXD may be related to three metabolic pathways: Gluconeogenesis, citrate cycle (TCA), acetyl entering Mitochondria, malate L-Aspartic acid shuttle and alanine metabolism.
张冉冉, 张艳, 杨秀东. 大剂量龙胆泻肝汤治疗氧化性肝损伤引发肝毒性的代谢组学机制[J]. 吉林化工学院学报, 2024, 41(9): 33-40.
ZHANG Ranran , ZHANG Yan , YANG Xiudong . Metabolomic Mechanism of Hepatotoxicity Induced by Oxidative Liver Injury Treated with High-dose Longdan Xiegan Decoction. Journal of Jilin Institute of Chemical Technology, 2024, 41(9): 33-40.
