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吉林化工学院学报, 2021, 38(9): 5-8     https://doi.org/10.16039/j.cnki.cn22-1249.2021.09.002
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吉林地区沙棘红茶活性成分及抗氧化活性的研究
冉丽梅,周鸿立*,刘宇琦,刘莹
吉林化工学院 化学与制药工程学院,吉林 吉林 132022
Study on the chemical constituents and antioxidant activity of black tea from Hipopphae rhamnoides L. leaves in Jilin Province
RAN Limei,ZHOU Hongli*,LIU Yuqi,LIU Ying
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摘要 

对沙棘叶及其制品红茶进行化学成分的研究,并对其抗氧化活性进行评价。方法:利用福林酚比色法、亚硝酸钠-硝酸铝-氢氧化钠显色法、苯酚硫酸法和国家标准方法《茶咖啡碱测定》,对沙棘茶中茶多酚、黄酮、多糖和咖啡碱含量进行分析;采用DPPH?、ABTS+、OH?和还原力4种方法进行抗氧化评价。结果:沙棘红茶主要化学成分:茶多酚为18.45%,黄酮含量为2.51%,多糖含量为15.56%,咖啡碱含量为3.06%;体外抗氧化实验结果表明,沙棘叶及其制品红茶对清除DPPH?、ABTS+ 、?OH自由基的IC50分别为2.47 mg/mL和0.76 mg/mL、0.0040 mg/mL和0.0038mg/mL、0.48mg/mL和0.41mg/mL,还原力与浓度有较好的剂量依赖关系。结论:由于沙棘红茶中含有有茶多酚、黄酮和多糖等活性成分因而有较好的抗氧化活性。

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冉丽梅
周鸿立
刘宇琦
刘莹
关键词:  沙棘茶  茶多酚  抗氧化活性     
Abstract: 

The chemical constituents and antioxidant potentials of Hipopphae rhamnoides L. leaves and its black tea product were investigated. Methods: The contents of tea polyphenols, flavonoids, polysaccharides and caffeine were analyzed by Folin colorimetric method, Sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method, Phenol-sulfuric acid method and national standard method of "Theophylline determination"; Four methods including DPPH?, ABTS+, ?OH and reducing power were used to evaluated antioxidant activity. Results: The main chemical components of the black tea: tea polyphenols was 18.45%, flavonoids content was 2.51%, polysaccharides content was 15.56% and caffeine content was 3.06%; the results of in vitro antioxidant experiments showed that the IC50 of Hipopphae rhamnoides L. leaves and its  black tea on scavenging DPPH?, ABTS+ and?OH free radicals were 2.47 mg/mL and 0.76 mg/mL, 0.0040 mg/mL and 0.0038 mg/mL, 0.48 mg/mL and 0.41 mg/mL, respectively. They also had a certain reducing power and a good concentration dependence. Conclusions: The antioxidant activity of black tea is better because of  active ingredients such as tea polyphenols, flavonoids and polysaccharides.

Key words:  Hipopphae rhamnoides L. leaves    tea polyphenols    antioxidant activity
               出版日期:  2021-09-25      发布日期:  2021-09-25      整期出版日期:  2021-09-25
S793.6  
引用本文:    
冉丽梅, 周鸿立, 刘宇琦, 刘莹. 吉林地区沙棘红茶活性成分及抗氧化活性的研究 [J]. 吉林化工学院学报, 2021, 38(9): 5-8.
RAN Limei, ZHOU Hongli, LIU Yuqi, LIU Ying. Study on the chemical constituents and antioxidant activity of black tea from Hipopphae rhamnoides L. leaves in Jilin Province . Journal of Jilin Institute of Chemical Technology, 2021, 38(9): 5-8.
链接本文:  
https://xuebao.jlict.edu.cn/CN/10.16039/j.cnki.cn22-1249.2021.09.002  或          https://xuebao.jlict.edu.cn/CN/Y2021/V38/I9/5
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