Melamine is an important kind of nitrogen heterocyclic organic chemical raw materials, the formula for nitrogen content is approximately 66%, widely used in wood, paper, textile, leather and other industries, can also be flame retardant, cleaner water reducing agent, formaldehyde, etc., but as a result of food and feed industry protein content test method of the defect, and melamine and tasteless is not easy to find, so often be unscrupulous businessmen used as food additive, to enhance the protein content of food testing index, the human body after the long-term or repeated intake of melamine can cause damage to the urinary system, kidney stones and other serious complications.Therefore is necessary to accurately detect melamine, this paper synthesized a kind of polymer point/gold nanoparticle cluster ratio fluorescent probes, melamine could be applied to physical testing, the results show that the fluorescence probe for melamine selectivity and good anti-interference, in 0 ~ 20 μM has good linear relationship, the detection limit of 0.23 μM.