The effects of the feeding time and feeding quality on the production of fuel ethanol by pulp of corn stalk fermentation are investigated by single factor experiment. According to the single factor test, a mathematical model of ethanol production from pulp of corn stalk fermented ethanol was established by response surface methodology. According to this model, the optimization of process parameters is carried out. The conversion rate of ethanol is taken as the index, the best enzymolysis conditions are obtained, under these enzymolysis conditions, the ethanol concentration of pulp of corn stalk after fermentation is 9.96 g/L at the conditions of the feeding time 50.19 h and feeding quality 6.35 g. The ethanol concentration of ethanol reached is 9.91±0.31g/L. Under the optimal conditions, the model is proved to be valid by the verification test. The results of this optimal technology will provide a reference for the ethanol production technology .