A method was developed for the separation of total flavonoids, total saponins, anthocyanins and polysaccharides from Aronia melanocarpa, and their antioxidant activity, inhibition of tyrosinase

activity and inhibition of α-glucosidase activity were investigated also. Using quality of target components as evaluation index, the optimal extraction parameter of total flavonoids, total saponins, anthocyanins and polysaccharides were ascertained as follows, the most suitable solvents as anhydrous ethanol, 60% ethanol, 60% ethanol and water, the absorption rate as 1.5, 2.5, 2.5, 2.5 mL/g, and the soaking equilibrium time as 2, 2, 2, 1 h, respectively. IC50 value was used as index to evaluate the activity difference in vitro between each component. The results showed during the first round of cyclic extraction the extraction rates of total flavonoids, total saponins, anthocyanins and polysaccharides were 76.09%, 84.38%, 73.75% and 72.23%, which that of all components reached more than 90% during the fourth round. The concentration of total flavonoids, total saponins, anthocyanins and polysaccharides in crude products were 8.93±0.16 mg/g, 70.39±0.26 mg/g, 1.51±0.02 mg/g and 695.46±1.39 mg/g, respectively, and they were purified to the concentration of 30.29±0.24 mg/g, 252.78±0.73 mg/g, 3.66±0.24 mg/g and 884.62±2.03 mg/g, respectively. Experiments on activity in vitro indicated that all the components had certain antioxidant activity, tyrosinase activity inhibition and α-glucosidase activity inhibition ability. Column chromatographic method combined with cyclic extraction could separate efficiently multiple active components which all possess different activities in vitro. The research results provided experimental basisand technical support for Aronia melanocarpa in food development and utilization field.