ZrO2/Na-β and ZrO2/H-β catalysts which ZrO2 supported on Na-β and H-β molecular sieves were prepared by impregnation method, and applied to the MPV reduction reaction of cyclohexanone and isopropanol to examine their catalytic properties. The catalysts were also characterized by XRD, SEM-EDS, NH3-TPD, BET specific surface area and pore structure, and ICP-MS. The products were identified by gas chromatography-mass spectrometer and analyzed and gas chromatography. The experimental results showed that ZrO2/H-β exhibited higher catalytic activity compared with H-β, Na-β, ZrO2/Na-β for the MPV reduction reaction of cyclohexanone and isopropanol. Due to ZrO2/H-β possesses both Zr and Al acid centers, these promote that the catalytic efficiency of MPV reaction of cyclohexanone and isopropanol was further improved. 6%ZrO2/H-β was used as the catalyst, the molar ratio of cyclohexanone to isopropanol was 1:20, and the dosage of catalyst was 6% of the total mass of the reactants. The reaction was carried out at 80 ℃ for 5 h. Based on cyclohexanone, the Area-normalized calculation, the conversion rate of cyclohexanone was 91.2%. The catalyst can be regenerated by calcination. The conversion rate of cyclohexanone decreases to 79.4% after 5 times of recycle, while the dried catalyst loses part of its activity after 1 time of recycle. The service life of the catalyst needs further study to improve.